Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNKRRNRQIVVAVNGGKAVKAIFLFIVSLIVIFVLSGVLTSLRPELRPSSDSFYGIAEELPGDVFAHLLQMENHYFASDLSQTDSSFHLSRLSLKLATSINLEDPRSFLGRELPGFAQFDTEILLAGQGTDYTNMPAESPPPSKVMEEEREANLAEIEKQQTQSDNAQKDPPKQTTGDKKVVFIYHTHNTESYLPLLKGETDPDMARHSKANVTLVGDMFGQALES-QGIGATVNKTDIQSKLNKKGLNYARSYDESRPVVKDALASNKNLQYIIDIHRDSRRKKDTTATIKGKSYARVAFVVGKKSKNFEENYKIASELHKLMEKKYPGLSTGVFSKGSPGDNGVYNQDLTDRALLLEFGGVDNNLEEL--------QRAANAAADVFSEMYWDAEKVNAASGETKKQ 1JWQ Chain:A ((30-177)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLAMALKVESILKQNPKLEVVLTRSDDTF--------------LELK-QRVKVAENLKANVFVSIHANSSGSSAS----NG---TET-YYQR------SASKAFANVMHKYFAPATGLTDRGIRYGN----FHVIRE-TTMPAVLLEVGYLSNAKEEATLFDEDFQNRVAQGIADGITEYLD---------------

General information: TITO was launched using: RESULT: Template: 1JWQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 636 24431 38.41 175.76 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 38.41 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_1JWQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JWQ-query.scw PDB file : Tito_Scwrl_1JWQ.pdb: