Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNYIPCMITIGNFICGLLAIHSLLYHNIHSAVLFIFTGMFLDFFDGMAARKLNAVSDMGRELDSFADLVTFGVAPSMLAYSVALYTLPFIGILCALTYSICGMLRLSKFNIEQSKLPTFIGMPIPFAGMCLVILSFTYNPILLAIGTCGLSYLMVSKIKFPHFKKHAAENLESGRWN-- 4IUM Chain:A ((1-127)) -GYNP----PGDGACGYRCLAFMNGATVVSA--GCSSDLWCD--DELAYRVFQLSPTFTVTIPGG------RVCPN------AKYAMICDK---------------QHWRVKRAKGVGLCLDESCFRGIC----------------NCQRMSGPPPAPVSAAVLDHILEAATFGNVRVV

General information: TITO was launched using: RESULT: Template: 4IUM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 641 -83306 -129.96 -666.44 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -129.96 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.164

(partial model without unconserved sides chains): PDB file : Tito_4IUM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IUM-query.scw PDB file : Tito_Scwrl_4IUM.pdb: