Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHLSILDQAPVSKGESPVTTLQHSVELAQLSEQWGYKRYWFAEHH---STKGLASTAPEIMIARIAAQTNTIRVGSGGVLLP-QYSPFKVAETFRQLEALYPNRIDLGVGRSPGGTTKTRLALTDGVKKSLTEFNRQLQDVSYFLTDSLPPDH-----PYAGIKAAPLIGTAPELWVLGLGENSARRAAHQGIGYVFGHFINPERGENAFRIYRESFRPSAHFSNPSALFTIFVICAKTDEEAEELALSQDLWLLRVGKGLDSRVPSIEEAKAHPYTASDKKLIEENRKRMVIGSPTTVKQQLLDLTGCYETNEIMVLCNVFDFEAKKESYERLAELFL- 1RHC Chain:A ((1-330)) -MKTQIGYFASLEQ-----YRPMDALEQAIRAEKVGFDSVWVDDHFHPWYHDNAQSAQAWAWMGAALQATKKVFISTCITCPIMRYNPAIVAQTFATLRQMYPGRVGVAVGAGEAMNEVPVTGEWPSVPVRQDMTVEAVKVMRMLWESDKPVTFKGDYFTLDKAFLYTKPDDEVPLYFSGMGPKGAKLAGMYGDHLMTVAAAPSTLKNVTIPKFEEGAREAGK--DPSKMEHAMLIWYSVDPDYDKAVEALRFWAGCLVPSMFKYKVYDPKEVQLHANLVHCDTIKENY--MCATDAEEMIKEIERFKEA-GINHFCLGNSSPDVNFGIDIFKEVIPAVRD

General information: TITO was launched using: RESULT: Template: 1RHC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1795 -160880 -89.63 -502.75 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -89.63 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_1RHC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RHC-query.scw PDB file : Tito_Scwrl_1RHC.pdb: