Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMSNQTVYQFIAENQNELLQLWTDTLKELSEQESYQLTDQVYENISKEYIDILLLSVKDENAAESQISELALRAVQIGLSMKFLATALAEFWKRLYTKMNDKRLPDQESTELIWQIDRFFSPINTEIFNQYSISWEKTVSLQKIALQELSAPLIPVFENITVMPLVGTIDTERAKRIMENLLNGVVKHRSQVVLIDITGVPVVDTMVAHHIIQASEAVRLVGAKCLLAGIRPEIAQTIVNLGIDLSQVITKNTLQKGIQTALEMTDRKIVSLGE 1SBO Chain:A ((10-110)) -----------------------------------------------------------------------------------------------------------------------------------------------------------EQDDKAIVRVQGDIDAYNSSELKEQLRNFISTTSKKKIVLDLSSVSYMDSAGLGTLVVILKDAKINGKEFILSSLKESISRILKLTHLDK-IFKITDTVEEA-----------------

General information: TITO was launched using: RESULT: Template: 1SBO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 442 -69795 -157.91 -691.04 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -157.91 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.650

(partial model without unconserved sides chains): PDB file : Tito_1SBO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SBO-query.scw PDB file : Tito_Scwrl_1SBO.pdb: