Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLKWRTVNPEETKAIAKLTAAFAKPGDVLTLEGDLGAGKTTFTKGFAEGLGITRIVNSPTFTIIKEYNDGVLPLYHMDVYRMED--ESEDLGLDEYFHGQGVCLVEWAHLIEEQLPQERLQIVIKRAGDDEREITFTAVGNRYEMLCEELSRHDNISN 1FL9 Chain:C ((38-150)) -----------------------------MVYLNGDLGAGKTTLTRGMLQGIGHQGNVKSPTYTLVEEYNIAGKMIYHFDLYRLADPEELEFMGIRDYFNTDSICLIEWSEKGQGILPEADILVNIDYY-DDARNIELIAQTN-----------------

General information: TITO was launched using: RESULT: Template: 1FL9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 479 -55377 -115.61 -498.89 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -115.61 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.282

(partial model without unconserved sides chains): PDB file : Tito_1FL9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FL9-query.scw PDB file : Tito_Scwrl_1FL9.pdb: