Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDTDLQILSHLQRNGRLTMVELGKLVGLSSPSAAERVRKLEDKGVITGYSANICYEKLNKHVTAFILMEPKSCKH--YAAFATSHPDVAENHRITGMYSYVTKVVTESVHTLEDFIDTSMAHGKPTTLVVLSSSSCHPAF 2ZNZ Chain:F ((25-142)) LDEIDKKIIKILQNDGKAPLREISKITGLAESTIHERIRKLRESGVIKKFTAIIDPEALGYSMLAFILVKVKAGKYSEVASNLAKYPEIVEVYETTGDYDMVVKIRTKNSEELNNFLD-----------------------

General information: TITO was launched using: RESULT: Template: 2ZNZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 331 -54409 -164.38 -469.04 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain F : 0.73 3D Compatibility (PKB) : -164.38 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_2ZNZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZNZ-query.scw PDB file : Tito_Scwrl_2ZNZ.pdb: