Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKELFVNLTILITFNYLFTHLFKERLVHKKDSISFQAVKGLACGLLGVILMVFGFTYQHSIIDLRNIPIMIAALYGGWVSTATALAMITAGRLLITMNTSALYSVIIICIAAIPSLIVSRRKKVQLKHAFYLLIITNSLISFSFYFLIDLHSYELHLYFWIISIAGGMLSLYIIDHETNAHLLFKQYKFQAHFDFLTGVYNRRKFEETTKALYQQAADTPHFQFALIYMDIDHFKTINDQYGHHEGDQVLKELGLRLKQTIRNTDPAARIGGEEFAVLLPNCSLDKAARIAERIRSTVSDAPIVLTNGDE-LSVTISLGAAHYPNNTEQPGSLPILADQMLYKAKETGRNRVCFSEKKE 1W25 Chain:B ((291-449)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DQLTGLHNRRYMTGQLDSLVKRATLGGDPVSALL-IDIDFFKKINDTFGHDIGDEVLREFALRLASNVRAIDLPCRYGGEEFVVIMPDTALADALRIAERIRMHVSGSPFTVAHGREMLNVTISIGVSATAGEGDTPEALLKRADEGVYQAKASGRNAVV------

General information: TITO was launched using: RESULT: Template: 1W25.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 743 -100202 -134.86 -634.19 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : -134.86 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.465

(partial model without unconserved sides chains): PDB file : Tito_1W25.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W25-query.scw PDB file : Tito_Scwrl_1W25.pdb: