Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLVSKLEETASEKPDSIACRFKDHMMTYQELNEYIQRFADGLQEAGMEKGDHLALLLGNSPDFIIAFFGALKAGIVVVPINPLYTPTEIGYMLTNGDVKAIVGVSQLLPLYESMHESLPKVE--LVILCQTGEAEPEAADPEVRMKMTTFAKILRPTSAAKQNQEPVPDDTAVILYTSGTTGKPKGAMLTHQNLYSNANDVAG-YLGMDERDNVVCALPMFHVFCLTVCMNAPLMSGAT-VLIEPQFSPASVFKLVKQQQATIFAGVPTMYNYLFQHENGKKDDFSSIRLCISGGASMPVALLTAFEEKFGVTILEGYGLSEASPVTCFNPFDRGRKPGSIGTSILHVENKVVDPLGRELPAHQVGELIVKGPNVMKGYYKMPMETEHALKDGWLYTGDLARRDEDGYFYIVDRKKDMIIVGGYNVYPREVEEVLYSHPDVKEAVVIGVPDPQSGEAVKGYVVPKR----SGVTEEDIMQHCEKHLAKYKRPAAITFLDDIPKNATGKMLRRALRDILPQ 2V7B Chain:B ((50-528)) ---------------------------TYGELEERARRFASALRTLGVHPEERILLVMLDTVALPVAFLGALYAGVVPVVANTLLTPADYVYMLTHSHARAVIASGALV---QNVTQALESA-GCQLIVSQP------LA--------PLFEELIDAAAPAAKAAATGCDDIAFWLYS-----KPKGTVHTHANLYWTAELYAKPILGIAENDVVFSAAKLFFAYGLGNGLTFPLSVGATAILMAERPTADAIFARLVEHRPTVFYGVPTLYANMLVSPNLPARADVAIRICTSAGEALPREIGERFTAHFGCEILDGIGSTEMLHIFLSNRAG-AVEYGTTGRPVPGYEIELRDEAGHAVPDGEVGDLYIKGPSAAVMYWNNREKSRATFLGEWIRSGDKYCRLPNGCYVYAGRSDDMLK----YVSPVEVEMVLVQHDAVLEAAVVGV-DHGGLVKTRAFVVLKREFAPSEILAEELKAFVKDRLAPHKYPRDIVFVDDLPKTATGKIQRFKLRE----

General information: TITO was launched using: RESULT: Template: 2V7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2684 -330114 -122.99 -728.73 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -122.99 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_2V7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V7B-query.scw PDB file : Tito_Scwrl_2V7B.pdb: