Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDFSLELPVRTNKPRETGQSILIDNGYPLQFFKDAIAGASDYIDFVKFGWGTSLL--TKDLEEKISTLKEHDITFFFGGTLFEKYVSQKKVNEFHR----YCTYFGCEYIEISNGTLPMTNKEKAAYIADFSD-EFLVLSEVGSKDAELASRQSSEEWLEYIVEDMEAGAEKVITEARESGTGGICSSSGDVRFQIVDDIISSDIDINRLIFEAPNKTLQQGFIQKIGPNVNLANIPFHDAIALETLRLGLRSDTFFL 3EWW Chain:A ((31-149)) ----------------ETNLCLSADVSL-ARELLQLADALGPSICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFENRKFADIG---NTVKKQYEGGIFKIAS-WADLVNAHVVPG---SGVVKGLQEVGLPLHRGCLLIA--------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3EWW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 479 -74504 -155.54 -665.21 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -155.54 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_3EWW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EWW-query.scw PDB file : Tito_Scwrl_3EWW.pdb: