Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKCARLNDRIIHLHTYSREHYQFLFEEGIKGHLFCSHCGKPVLLRLNIADPPEFIHRQPGDFPACEEACEPKPSKEGKKEDDQESGVIRLPKGKAIAADPSPAVTEWHRPRSIKPGTPFVPKTIEPDTSLFPSVGLNTDQLKAVTETEGPLLVLAGAGSGKTRVLTARAAHMIEHLGIPPENMLLVTFTTKAVAEMKERMANQYGLQPAKVRRIVTGTFHSLFYKILYHSNSAKWNGEHL-------LKMEWQREQYIKKALYE-EGIDEKESP------VDQALQQIGFWKNTYVPNERIPLKDEWEKQVY-RLYEHYERQKKEHSQFDFDDMASACYELFIERPDLLEQYQSRFTYILIDEFQDINPVQYKIMQMLASPEQNLCCVGDDDQSIYAFRGSNPSFILDFQKDYPGAKTIYLTANYRSTHPIVSSADIVVKKNKNRYAKTLEAARDDIQVPVLFY-PYDEEEEATMVVSDIKEKIQNGASPEDFAVLYRTNSGGRAIYERLHQSSIPYTADRGVQSFYSRRIVRQILAYLYASQNEDDTEAI--KHLLPALFLKQSALNTLKALSITEDCTMIKALAKLPDLKPFQLDKIKKIVPFFASLR--------TMKPVEAITFAEGKMGFSEYLKKRGNEG-----------NKLEKGSDDLRDIKVVAKKFKTIPDFLAHVDHMRAAEKNRTDEHGVQLMTIHRSKGLEFKTVYVLGTVDGSIPHDFSLETARKGDEAALEEERRLLYVAMTRAKQHLYLSCPANRR--GKT--ANRSRFLYPLLQKARQPLHH 4C30 Chain:I ((13-645)) ---------------------------------------------------------------------------------------------------------------------------------------LNPTQAQAADHFTGPALVIAGAGSGKTRTLIYRIAHLIGHYGVHPGEILAVTFTNKAAAEMRERAGH---LVPG-AGDLWMSTFHSAGVRIL------RTYGEHIGLRRGFVIYDDDDQLDIIKEVMGSIPG---ETQPRVIRGIIDRAKSNL--WTPDDLDRSREPFISGLPRDAAAEAYRRYEVRKKGQNAIDFGDLITETVRLFKEVPGVLDKVQNKAKFIHVDEYQDTNRAQYELTRLLASRDRNLLVVGDPDQSIYKFRGADIQNILDFQKDYPDAKVYMLEHNYRSSARVLEAANKLIENNTERLDKTLKPVKEAGQ-PVTFHRATDHRAEGDYVADWLTRLHGEGRAWSEMAILYRTNAQSRVIEESLRRVQIPARIVG-----YDRREIRDILAYARLALNPADDVALRRIIGRPRRGIGDTALQKLMEWARTHHTSVLTACANAAEQNI--LDRGAHKATEFAGLMEAMSEAADNYEPAAFLRFVMETSGYLDLLRQEGQEGQVRLENLEELVSAAEEWSQDEA---GSIADFLDDAALLSSVDD-------GAPEDAVTLMTLHNAKGLEFPVVFIVGVEQGLLPSKGAIAEGPSG----IEEERRLFYVGITRAMERLLMTAAQNRMQFGKTNAAEDSAFL-------------

General information: TITO was launched using: RESULT: Template: 4C30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 2999 -237469 -79.18 -413.71 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain I : 0.73 3D Compatibility (PKB) : -79.18 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_4C30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C30-query.scw PDB file : Tito_Scwrl_4C30.pdb: