Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALFTAKVTARGGRAGHITSDDGVLDFDIVMPN-AKKEGQTGTNPEQLFAAGYAACFGGALEHVA--KEQNIEIDSEIEGQVSLMKDESDGGFKIGVTLVVNTKDLDREKAQELVNAAHEFCPYSKATRGNVDVKLELK 1N2F Chain:B ((6-141)) -ALYTATATATGGRDGRAVSSDGVLDVKLSTPREMGGQGGAATNPEQLFAAGYSACFIGAMKFVAGQRKQTLPADASITGKVGI--GQIPGGFGLEVELHINLPGMEREAAEALVAAAHQVCPYSNATRGNIDVRLNVS

General information: TITO was launched using: RESULT: Template: 1N2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 521 -58534 -112.35 -440.10 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -112.35 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_1N2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N2F-query.scw PDB file : Tito_Scwrl_1N2F.pdb: