Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------MKKTFVKKAMLTTAAMTSAALLTFGPDAASAKTPVDNTAVQLQHQ----ASTNEDLNTFI----DILNQCIYEQDGVYYFDSEKAVEL-GMTKEEAQVIATLWEST-----SEFFSIVSQCVYLEDGNYKFDTEKAVELGFTEKEALALEQFFSAVSLKIHILQAAIVLQDDVYSYDKDAALQAGATPLQADVYEKLFSALSQEQLAAIYDMIHPQA------------ 4Q0W Chain:A ((1-362)) GVHSFWDIAGPTARPVRLESLEDKRMAVDASIWIYQFLKAVRDQNAVKNSHITGFFRRICKLLYFGIRPVFVFDGGVPVLKRETIRQRKERRQGKREDEVTMDMIKEVQ---ELLSRFGIPYITAPMEAEAQCAELLQLNLVDGIITDDSDVFLFGGTKIYKNMFHEKNYVEFYDAESILKLLGLDRKNMIELAQLLGSDYTNGLKGMGPVSSIEVIAEFGNLKNFKDWYNNGQFDKRKQETENKF--EKDLRKKLVNNEIILDDDFPSVMVYDAYMR--PEVDHDTTPFVWGVPDLDMLRSFMKTQLGWPHEKSDEILIPLI

General information: TITO was launched using: RESULT: Template: 4Q0W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 584 -27185 -46.55 -138.70 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -46.55 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_4Q0W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q0W-query.scw PDB file : Tito_Scwrl_4Q0W.pdb: