Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIKDLMTADLQYCTVLDNVYEAAVKMKDANVGAIPVVDEDGETLVGIVTDRDLVLRGIAIKKPNS--QKITDAMTEKPVSVEEDASVDEVLHLMASHQLRRIPVTKNKKLTGIVTLGDLSLSEQTNERAGSALSDISEGDNREEGFFH 3FHM Chain:D ((45-144)) -------------------SIGEAAGTLHAHKIGAVVVTDADGVVL-GIFTERDLV-KAVAGQGAASLQQSVSVAMTKNVVRCQHNSTTDQLMEIMTGGRFRHVPVE--GRLAGIISIGDV-----------------------------

General information: TITO was launched using: RESULT: Template: 3FHM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 322 -51109 -158.72 -532.38 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain D : 0.76 3D Compatibility (PKB) : -158.72 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_3FHM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FHM-query.scw PDB file : Tito_Scwrl_3FHM.pdb: