Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELNKTKVRKELRTERPSIYSFELDEIKQWLTDNGEKPFRAAQIFEWLYEKRVSSFEDMTNLSKDLREKLNTRFVLTTLKTAVKQTSQDGTMKFLFELHDGYTIETVLMRHEYGNSVCVTTQVGCRIGCTFCASTLGG--------LKRNLEAGEIVAQVVKVQKALDETDERVSSVVIMGIGEPFDNFNEMLAFLKIINHDKGLNIGARHITVSTSGIIPK--IYEFADQQMQINFAISLHAPNTEIRSRLMPINRAYKLPDLMEAVKYYINKTGRRISFEYGLFGGVNDQVEHAEELADLLEGVKCHVNLIPVNYVPERDYVRTPRDQIFAFEKTLKSRGVNVTIRREQGHDIDAACGQLRAKERQDETR 1TV8 Chain:A ((14-201)) -------------------------------------------------------------------------------------------------------------------RDLRLSVTDRCNFRCDYCMPKEVFGDDFVFLPKNELLTFDEMARIAKVYAE------LGVKKIRITG-GEPLMRRD-LDVLIAKLNQIDGI----EDIGLTTNGLLLKKHGQKLYD-AGLRRINVSLDAIDDTLFQSINNRNI--KATTILEQIDYATS-IGLNVKVNVVIQKGI--NDDQIIPMLEYFKDKHIEIRFIEFMDVGN----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1TV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 812 -125373 -154.40 -704.34 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -154.40 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_1TV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TV8-query.scw PDB file : Tito_Scwrl_1TV8.pdb: