Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITYVFPGQGSQKQGMGSGLFD--EFKELTDQADEILGYSIKRLCLENPYSNLNKTQFTQPALYVVNALSYLKKIRDEEVKPDFV------AGHSLGEYNALFAAEAFDFETGLQLVRKRGELMSLISNG---GMAAVMGLNEEQVAKALKEYHLHD---------VDIANVNAPYQIVISGKKDEI---EKAASLFETMTEVTMVLPLNVSGAFHSRYMNKAKEEFEEFLHAFYFSPPSIPVISNVYAKPYTYE-FMKQTLADQINHSVKWTDSIS--YLMKKGH--MEFEEVGPGNVLTGLIHRIKKDAEAMPR 2C2N Chain:A ((30-329)) ----LFPGQGSQVVGMGRGLLNYPRVRELYAAARRVLGYDLLELSLHGPQETLDRTVHCQPAIFVAS-LAAVEKL--HHLQPSVIENCVAAAGFSVGEFAALVFAGAMEFAEGLYAVKIRAEAMQEASEAVPSGMLSVLGQPQSKFNFACLEAREHCKSLGIENPVCEVSNYLFPDCRVISGHQEALRFLQKNSSKFHFRR--TRMLP--VSGAFHTRLMEPAVEPLTQALKAVDIKKPLVSVYSNVHGHRYRHPGHIHKLLAQQLVSPVKWEQTMHAIYERKKGRGFPQTFEVGPGRQLGAILKSCNMQA-----

General information: TITO was launched using: RESULT: Template: 2C2N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1488 -152056 -102.19 -559.03 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -102.19 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_2C2N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2C2N-query.scw PDB file : Tito_Scwrl_2C2N.pdb: