Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVQII-FDSKTGNVQRFVNKTGFQQIR-KVDEMDH--VDTPFVLVTYTTN----FGQVPASTQSFL--EKYAHLLLGVAASGNKVWGDNFAKSADTISRQYQVPILHKFELSGTSKDVELFTQEVERVVTKSSAKMDPVK 3N39 Chain:D ((23-139)) --QLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYILIVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCGVPWLYRFELMGTQSDIE---------------------

General information: TITO was launched using: RESULT: Template: 3N39.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 459 -60461 -131.72 -565.06 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.73 3D Compatibility (PKB) : -131.72 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_3N39.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N39-query.scw PDB file : Tito_Scwrl_3N39.pdb: