Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------MNRMSTIFLKIALVLIGIPILALCIFLVPKVANY-----SAELFPNIAYIKYLVFIYLYVTAIPFYFALYQAFKLL---------SYIDKNKAFSGLSVRALKNIKYCAVTISIFYAA---------GMPVFYLMAEID------------DAPGIIVIGLVIIFASMVIAVFAAVLQKLLKEAIDIKSENDLTV 3BEH Chain:A ((7-228)) LRIYAPLNAVLAAPGLLAVAALTIPDMSGRSRLALAALLAVIWGAYLLQLAATLLKRRAGVVRDRTPKIAIDVLAVLVPLAAFLLDGSPDWSLYCAVWLLKPLRDSTFFPVLGRVLANEARNLIGVTTLFGVVLFAVALAAYVIERDIQPEKFGSIPQAMWWAVVTLSTTGYGDTIPQSFAGRVLAGAVMMSGIGIFGLWAGILATGFYQEVRRGDFVRNWQ

General information: TITO was launched using: RESULT: Template: 3BEH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 396 -78652 -198.61 -491.57 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -198.61 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.191

(partial model without unconserved sides chains): PDB file : Tito_3BEH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BEH-query.scw PDB file : Tito_Scwrl_3BEH.pdb: