Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAGYLNNIALNLEIVLKNKADSPEVSETLVTRICENLLLSKEVSFLKADGSVENFKLSDMEYEITNTEELPE 2RF4 Chain:B ((16-101)) ---------LNTPVVIHATQLPQHVSTDEVLQFLESFIDEKENI--IDIDTNLSSSISQLKRIQRDFKGLPP

General information: TITO was launched using: RESULT: Template: 2RF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 77 -4104 -53.29 -67.27 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : -53.29 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_2RF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RF4-query.scw PDB file : Tito_Scwrl_2RF4.pdb: