Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTETTENVVITIPDKTSFTFHEAATSPSEGEEFVVGHFRELTVKISGSSTSREIKFYAVDENGEKTALSGTNKTDFQLGSSTLNTNEYWDFDIAGLFKVMFEVVSVTGDVTVKGIVVS 1QF6 Chain:A ((2-62)) -------PVITLPDGSQRHYDHAVS------------PMDVALDIG---PGLAKACIAGRVNGELVDACDLIENDAQLSIITA-----------------------------------

General information: TITO was launched using: RESULT: Template: 1QF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 247 -20239 -81.94 -331.79 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -81.94 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_1QF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QF6-query.scw PDB file : Tito_Scwrl_1QF6.pdb: