Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMAYEEYMRQLVVPMRRELTGAGFEELTTAEEVENFMEKAEGTTLVVV-NSVCGCAAG-LARPAATQAVLQNDKTPDNTVTVFAGQDKEATAKMREYFTGQEPSSPSMALLKGKEVVHFIPRHEIEGHDMEEIMKNLTAAFDAHC 2ES7 Chain:A ((11-123)) ----------------WQRLLTRGWQPVE-AS---------VGDGVILLSSDPRR----SDNPVMIAELLRE--FPQFD--WQVAVADLEQSEAIGDRFN--VRRFPATLVFTDG----ALS----GIHPWAELLTLMRSI-----

General information: TITO was launched using: RESULT: Template: 2ES7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 382 2596 6.79 27.32 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 6.79 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_2ES7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ES7-query.scw PDB file : Tito_Scwrl_2ES7.pdb: