Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEALASPNRFIGLIAEVKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPVLRKDF-IDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEK-GMDVLVEVHDASTLEQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKE---SLLVSESGIGSLEHLTFVNEHGARAVLIGESLMRQTSQRKAIHALFRE 1VC4 Chain:B ((11-252)) VLGEIARKRASEVAPYPLP--EPPSVPSFKEALLRPG--LSVIAEVKRQSPSEGLIRE-VDPVEAALAYARGGARAVSVLTEPHRFGGSLLDLKRVREAVDLPLLRKDFVVDPFMLEEARAFGASAALLIVALLG--ELTGAYLEEARRLGLEALVEVHTERELEIALEAGA-EVLGINNRDLATLHINLETAPRLGRLARKRGFGGVLVAESGYSRKEELKAL-EGLFDAVLIGTSLMRAPDLEAALREL---

General information: TITO was launched using: RESULT: Template: 1VC4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1349 -8340 -6.18 -35.19 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -6.18 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_1VC4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VC4-query.scw PDB file : Tito_Scwrl_1VC4.pdb: