Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLGIDMNVKESVLCIRLTGELDHHTAETLKQKVTQSLEKDDIRHIVLNLEDLSFMDSSGLGVILGRYKQIKQIGGEMVVCAISPAVKRLFDMSGLFKIIRFEQSEQQALLTLGVAS 1TID Chain:D ((5-119)) -SLAIDLEVKQDVLIVRLSGELDHHTAEELREQVTDVLENRAIRHIVLNLGQLTFMDASGLGVILGRYKQIKNVGGQMVVCAVSPAVKRLFDMSGLFKIIRVEADEQFALQALGVA-

General information: TITO was launched using: RESULT: Template: 1TID.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 557 -116411 -209.00 -1012.27 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.94 3D Compatibility (PKB) : -209.00 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.627

(partial model without unconserved sides chains): PDB file : Tito_1TID.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TID-query.scw PDB file : Tito_Scwrl_1TID.pdb: