Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLLMLTTGGTIASVEGENGLAPGVKADELLSYVSKLDN----DYTMETQSLMNIDSTNMQPEYWVEIAEAVKENYDAYDGFVITHGTDTMAYTSAALSYMLQHAKKPIVITGSQIPITFQKTDAKKNITDAIRFACE-GVGGVYVVFDGRVIQGTRAIKLRTKSYDAFESINYPYIAFINEDGIEYNKQVTEPE---NDTFTVDTSLCTDVCLLKLHPGLKPEMF-DALKSMYKGIVIESYGSGGVPFEGRDILSKVNELIESGIVVVITTQCLEEGEDMSIYEVGRRVNQDLIIRSRNMNTEAIVPKLMWALGQSSDLPVVKRIMETPIADDVVL 2P2N Chain:C ((24-346)) -KSIYVAYTGG-------------------QLALMPEFHRPEMPDFTIHEYTPL-MDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTTKAHADGFDAFASPNLPPLL---EAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILRSYGVGNAP-QNKAFLQELQEASDRGIVVVNLTQCMSGKVNM-------ALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAM----------

General information: TITO was launched using: RESULT: Template: 2P2N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1598 -4083 -2.55 -14.22 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -2.55 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_2P2N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P2N-query.scw PDB file : Tito_Scwrl_2P2N.pdb: