Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MITQLYRERT------AADLKNRISKVLLNGNETEIVELTIQGAVVTVLTQREEDIK---HIKSVQILDDQNNVITERTTDLDVSNNRTLDFRITFEVV----------------------------------------------------------------------------------------------------------------------------------------------------- 5CVL Chain:A ((27-578)) RRKVQVSYVIRDEVEKYNRNGVNALQLDPALNRLFTAGRDSIIRIWSVNQHKQDPYIASMEHHTDWVNDIVLCCNGKTLISASSDTTVKVWNAHKGFCMSTLRTHKDYVKALAYAKDKELVASAGLDRQIFLWDVNTLTALTASNNTVTTSSLSGNKDSIYSLAMNQLGTIIVSGSTEKVLRVWDPRTCAKLMKLKGHTDNVKALLLNRDGTQCLSGSSDGTIRLWSLGQQRCIATYRVHDEGVWALQVNDAFTHVYSGGRDRKIYCTDLRNPDIRVLICEEKAPVLKMELDRSADPPPAIWVATTKSTVNKWTLKG----IHNFRASGPITPLCTQPDQVIKGGASIIQCHILNDKRHILTKDTNN----NVAYWDVLKACKVEDLGKVDFEDEIKKRFKMVYVPNWFSVDLKTGMLTITLDESDCFAAWVSAKDAGFSSPDGSDPKLNLGGLLLQALLEYWPRTHVNPMDKGNGYFQVPPHTPVIFGEAGGRTLFRLLCRDSGGETESMLLNETVPQWVIDITVDKNMPKFN

General information: TITO was launched using: RESULT: Template: 5CVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 298 12588 42.24 153.51 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 42.24 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.196

(partial model without unconserved sides chains): PDB file : Tito_5CVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CVL-query.scw PDB file : Tito_Scwrl_5CVL.pdb: