Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDTAMARLN-----EGTIVIYALSVLFYFIDFLQHNRKA-------------GKMAFWLLSIVWTLQTVYLAYFMWVTGRFPVLNVTEALYFYAWVL----VTLSLVLTKLLRV---DFIVFFTNVIGFSMIAIHTFSPTEQQ----------SAAFSGQLVSELLVIHITMAILSY---------------GAF----SLSFV-FSVLYMFQYHLLK--KKKWGKWLLRIEDLSKLDYMAYVLNVIGVPMLL--LSLILGVIWAYVSLET-----LYWFDAKVLGSFVVLLLYSYYLYIRLIKELQGKVAALWNTACFLVLMIN--YFLLGSLSQFHWFS 4V12 Chain:A ((7-343)) DTSTLESRVGHYYQMEDTYLV-GREKVREFARAVQDYHPAHWNLATAADLGHPGLIAPLTFTSAPAMACNQRMFESVVVGYDMYLQTEEVFEQHRPIVEGDELSIDIELTSVRRIAGRDLITVTNTFTDTAGEVVHTLHTTVVGITAEDVDPAIRPAVQGVMMHGINMLGVEETNAPYEKTVRPEGELRIAQGGATRTPTSLNFDDLKVGEELPVHTARLSRGDLVNYAGVAGDANPLHWDENIAKLAGQPDVIAHGMLTMGLGAGFVSSWSGDPGAITRYAVRLSQPAVVPAEGTEIEYSGRIKSLDPET----RTGVVIVAAKSGGRKIFGLATATIRFS

General information: TITO was launched using: RESULT: Template: 4V12.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1245 -102522 -82.35 -378.31 target 2D structure prediction score : 0.25 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -82.35 2D Compatibility (Sec. Struct. Predict.) : 0.25 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.112

(partial model without unconserved sides chains): PDB file : Tito_4V12.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4V12-query.scw PDB file : Tito_Scwrl_4V12.pdb: