Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MTTEKEMKQIQNEFKERMDEGELLNCMRCGFCLPSCPTYIESGFQETHSPRGRIALMKAVADGMIEPDEDVERSLSLCLGCRACEPVCPSGVKYGQLLEEARDIIHQNKKHSLGERVMRKTAFHELFPHQNRMRSAVSLIGLYQRSGLQTAVRKSGMLRVLPEHLRTMEAVLPDVPKS-KDMKHRPRFLPSIGPMKKRVAFFSGCLMDTMFLPTNNATLKLLQLAGCDIVIPPEQACCGALHGHSGEKNTGKELAKQNIAAFEALDVDAVITNAGGCGAFLTEYDHLLKDDPEWSERAAAFVQKLKDFSSVLVE-LDFHQMDLALETPQVVTYQDSCHLRNVMHTSLEPRQLLKSIKGAEFKEMEKADSCCGSAGIYNIVEVEMSMKILDSKMAAVKATEAILIVTANPGCLLQMKLGIE----REGLSGKVRAVHLADLLLEAAGHKTS-- 4N5H Chain:X ((1-315)) MADEEAMLAKVQASWAQTAARDKARYAD-------------ERVPEDVHWETEYR----------YEQSADPQQTLNLYYPAKRRNATMPTVIDI----------------HGGGW----------FYGDRNLNRNYCRYLAS---QGY--AVMGMGY-RLLPD--VDLRGQIQDIFASLRWLSHF-------GPQR-------GFDLDHVLLTGDSAGGHLASLVACI-----------------QQSAELQELFGVSRVNFN-FTLVALV-------CPVAEPSKLPEAAGDMSDMAAFYLDKLSGGDQALVDHLNFSQV--------------------VKGLDLPPFMLI----GGQNDSF------------------YLQSQALLKVFDANHVTYTTKLWPASAGPHLKHVFNVQHWEWPESIETNLEMLRTFDALSKQQDQAEENE

General information: TITO was launched using: RESULT: Template: 4N5H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1743 -8551 -4.91 -27.94 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain X : 0.65 3D Compatibility (PKB) : -4.91 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.292

(partial model without unconserved sides chains): PDB file : Tito_4N5H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N5H-query.scw PDB file : Tito_Scwrl_4N5H.pdb: