Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLNDILEFNKTFTEQREYEKYQTSKFPDKKMAILSCMDTRLVELLPHAMNLRNGDVKIIKSAGALVTHPFGSIMRSILVAVYELNADEVCVIGHHDCGMSKISSKSMLEKIKARGIPEERIETIKYSGVDFDQWFKSFDSVEASVKDSVDVIKHHPLFPENVPVHGLVIDPKTGKLDLIVNGYNN 1G5C Chain:D ((2-163)) --IIKDILRENQD------------SP----KLCIITCMDSRLIDLLERALGIGRGDAKVIKNAGNIVDD---GVIRSAAVAIYALGDNEIIIVGHTDCGMARLDEDLIVSRMRELGVEEEVIENFSI------DVLNPVGDEEENVIEGVKRLKSSPLIPESIGVHGLIIDINTGRL---------

General information: TITO was launched using: RESULT: Template: 1G5C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 654 -115207 -176.16 -762.96 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.79 3D Compatibility (PKB) : -176.16 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.663

(partial model without unconserved sides chains): PDB file : Tito_1G5C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G5C-query.scw PDB file : Tito_Scwrl_1G5C.pdb: