Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKICVVGAGYVGLTLSAALASIGHDMICTDKDVKKIGQLKKGVIPFYEPGLSDAILR---CGNLSFSSEVKSSMEECPVIFIAVGTPPRSDGSADTKALQSVIGDLSEAIRSYKTIITKSTVPPGTNENI----AKQLIASGVSKNLFNIVSNPEFLREGNALYDMLHPDKTVIGVQEEDHVSAAIVKSIYKHIDTPF-------IVTSLAGAELIKYANNFFLAAKISFINEMARICEAYQSDISDISRAIGLDPRIGKHFLQAGIGYGGSCFPKDLQALQFAAQEKNTETFLLRAVQHINDTQLGLYIKKIQSFF-ETLQGKKAAVLGISFKPNTDDIRNSQAVRLMERLAELGCDVHAYDPEA------VLPEHLRQH---------VTQHSQAFDAIEESDFLFLATEWSEFLAFDWKKAADIMKGRLVIDGRNVLKKELIEACGLICTGVGRP 2Y0E Chain:D ((9-460)) MNLTIIGSGYVGLVTGACLADIGHDVFCLDVDQAKIDILNNGGVPIHEPGLKEVIARNRSAGRLRFSTDIEAAVAHGDVQFIAVGTPP------DLQYVLAAARNIGRYMTGFKVIVDKSTVPVGTAERVRAAVAEELAKRG-GDQMFSVVSNPEFLKEGAAVDDFTRPDRIVIGC--DDDVPGERARELMKKLYAPFNRNHERTLYMDVRSAEFTKYAANAMLATRISFMNELANLADRFGADIEAVRRGIGSDPRIGYHFLYAGCGYGGSCFPKDVEALIRTADEHGQSLQILKAVSSVNATQKRVLADKIVARFGEDLTGRTFAIWGLAFKPNTDDMREAPSRELIAELLSRGARIAAYDPVAQEEARRVIALDLADHPSWLERLSFVDDEAQ---AARDADALVIVTEWKIFKSPDFVALGRLWKTPVIFDGRNLYEPETMSEQGIEYHPIGRP

General information: TITO was launched using: RESULT: Template: 2Y0E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2151 -9087 -4.22 -21.84 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -4.22 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_2Y0E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y0E-query.scw PDB file : Tito_Scwrl_2Y0E.pdb: