Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGFY--NSGGYSGNSGYSNGFGSSFALIVVLFILLIIVGAAIFNY 2K05 Chain:B ((101-138)) MEGISITSDNTEEMGSGDDSMKEP----------AFREENANFNK

General information: TITO was launched using: RESULT: Template: 2K05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 5 282 56.30 8.53 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.48 3D Compatibility (PKB) : 56.30 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.48 QMean score : -0.232

(partial model without unconserved sides chains): PDB file : Tito_2K05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2K05-query.scw PDB file : Tito_Scwrl_2K05.pdb: