Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRVLLIDDHEMVRMGLAAFLEAQPDIEVIGEASDGSEGVRLAVELSPDVILMDLVMEGMDGIEATKQICRELSDPKIIVLTSFIDDDKVYPVIEAGALSYLLKTSKAAEIADAIRAASKGEPKLESKVAGKVLSRLRHSGENALPHESLTKRELEILCLIAEGKTNKEIGEELFITIKTVKTHITNILSKLDVSDRTQAAVYAHRNHLVN 4IF4 Chain:B ((2-208)) -IKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKAHELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSYILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAE---LYEMLTEREMEILLLIAKGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ

General information: TITO was launched using: RESULT: Template: 4IF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 953 -125703 -131.90 -607.26 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.88 3D Compatibility (PKB) : -131.90 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_4IF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IF4-query.scw PDB file : Tito_Scwrl_4IF4.pdb: