Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAESLLIKDIAIVTENEVIKNGYVGINDGKISTVSTERPKEPYSKEIQAP--ADSVLLPGMIDIHIHGGYGADTMDA-SFSTLDIMSSRLPEEGTTSFLATTITQEHGNISQALVNAREWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVELFKKWQQEAGGLIKIVTLAPEEDQHFELIRHLKDESIIASMGHTDADSALLSDAAKAGASHMTHLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITDSMRAKGLKDGVYEFGGQSVTVRGRTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIANITSENAAKQLGIFDRKGSVTVGKDADLVIVSSDCEVILTICRGNIAFISKEADQI 3IV8 Chain:C ((7-376)) ------LTNCKIYTGNDVLVKHAVIINGDKIEAVC---PIESLPSEMNVVDLNGANLSPGFIDLQLNGCGGVMFNDEITAETIDTMHKANLKSGCTSFLPTLITSSDENMRQAIAAAREYQAKYPN-----QSLGLHLEGPYLNVMKKGIHSVDFIRPSDDTMIDTICANSDVIAK-VTLAPENNKP-EHIEKLVKAGIVVSIGHTNATYSEARKSFESGITFATHLFNAMTPMVGREPGVVGAIYDTPEVYAGIIADGFHVDYANIRIAHKIKG-EKLVLVTDATAPAGAEMDYFIFVGKKVYYRDGKCVDENGTLGGSALTMIEAVQNTVEHVGIALDEALRMATLYPAKAIGVDEKLGRIKKGMIANLTVFDRDFNVKATVVNG------------

General information: TITO was launched using: RESULT: Template: 3IV8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2340 13589 5.81 37.03 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : 5.81 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_3IV8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IV8-query.scw PDB file : Tito_Scwrl_3IV8.pdb: