Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVAIASDHGGVHIRNEIKELMDELQIEYIDMGCDCGSGSVDYPDYAFPVAEKVVSGEVDRGILICGTGIGMSISANKVKGIRCALAHDTFSAKATREHNDTNILAMGERVIGPGLAREIAKIWLTTEFTGGRHQTRIGKISDYEEKNL 3PH4 Chain:B ((21-166)) MKIGIGSDHGGYNLKREIADFLKKRGYEVIDFGTH-GNESVDYPDFGLKVAEAVKSGECDRGIVICGTGLGISIAANKVPGIRAAVCTNSYMARMSREHNDANILALGERVVGLDLALDIVDTWLKAEFQGGRHATRVGKIGEIEKK--

General information: TITO was launched using: RESULT: Template: 3PH4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 772 -104146 -134.90 -713.33 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.91 3D Compatibility (PKB) : -134.90 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_3PH4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PH4-query.scw PDB file : Tito_Scwrl_3PH4.pdb: