Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSNQNNDIKTKHHFPLLLALALTMGVFAAGSEELVISPLLPDLAKAFS--------------SDVSVLALSISIYGVMIFIGAPLLVPLGDKYSRELSLLAGLMIFIIGTVICAL---AQNIFFFFLGRALSGLAAGAFVPTAYAVVGDRVPYTYRGKVMGLIVSSWSLALIFGVPLGSFIG--GVLHWRWTFWIFALMGVLVVLLILLEMRRHAQHKN---SGKE-E---------------------------IEEPAGTFRDALKVPRVPVYITITFCNMIG-----FYGMYSFLGTYLQDVFTGGNTAAGLFIMIYGIG-FSMSVITGKIADRIGKMRSLLIALGVISVLLACLPYAP-------A-SMFLLIASLFIWGLMQSLTVTLLSTILSDCSE-RHRGKVMVFYSLASNLAVTLGSALMGPVYVAYGYAAVGLICAAITVLGFVLSVFAYKKYGKLEQKADQSLSQ 4ZW9 Chain:A ((31-457)) --------------ALIFAITVATIGSFQFGYNTGVINAPEKIIKEFITKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSAA-LPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKD-A--GVQEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLGAYVFIIF--------------------------------

General information: TITO was launched using: RESULT: Template: 4ZW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1519 -187081 -123.16 -516.80 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -123.16 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_4ZW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZW9-query.scw PDB file : Tito_Scwrl_4ZW9.pdb: