Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIIMGVFTTIVNIASFYILVEIMNVDYKAATVAAW-------------------------------ILSVLFAYITNKLYVFQQKTHDLQSLLKELTAFFSVRVLSLGIDLGMMIILVGQFNTNETLAKILDNAVIVVVNYVASKWLVFKKTKEEGV-------------------------------- 4G4S Chain:P ((3-269)) MSCLVLPLVSVGNIPQLSIDWLLNSQANEWEYLEALDSKYLVEFVGPLDRPEDGSDSLYKDADMKYSSALEVFYNKKRGLFAIQQRTPLVSVNYLNNFIVEIILPFLSKYNISEICIWDSLYAMVDQPIFKILFQSIKYCSCLANEGDNSLDSQQFLQWIISQVKFVRPISWQGAYGMADARDKFVDLYN

General information: TITO was launched using: RESULT: Template: 4G4S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 503 -72269 -143.68 -569.05 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain P : 0.56 3D Compatibility (PKB) : -143.68 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_4G4S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G4S-query.scw PDB file : Tito_Scwrl_4G4S.pdb: