Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKDKIRVALADDQPLVREGFRYVINAQTDMTVSGEAGDGHDIIALAKQTKPDVILMDVQMPRCSGIEAAKDIMSALPNTKIVILTTFDTEEYVFEGIRAGAVGYLLKDTLPEELIDAIRAAARGEAIFRTVTAAKIISETFRAKQQTHAEELAEPFTKRELEVLQQMAYGLRNEDIAEKLFVSESTVKTHVHRILQKCNAQDRTQAVVFAIRNGIVQ 4IF4 Chain:B ((2-208)) -----IKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKAHELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSYILKTTSAKDIADAVRKTSRGESVFEPEVLVKM-----RNRMKKRA-ELYEMLTEREMEILLLIAKGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ

General information: TITO was launched using: RESULT: Template: 4IF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 953 -129936 -136.34 -627.71 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -136.34 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_4IF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IF4-query.scw PDB file : Tito_Scwrl_4IF4.pdb: