Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQQLKDIISACDLAIQNEDFDTLMNYYSEDAVLVVKP--GMIARGKEEIKKAFITIANYFNHHIVPTQGKMILLEAG-DTVLVLS-QTLLDSDKKDSEYAMERRATYVFKKNAQGEWLCVIDNSYGTDLIGV 2F86 Chain:B ((350-471)) -KQDIVRVTQTLLDAISCKDFETYTRLCDTSMTCFEPEALGNLIEGIEFHRFYFDGNRKNQ---VHTTMLNPNVHIIGEDAACVAYVKLTQFLDRNGEAHTRQSQESRVWSKK-QGRWVCVHVHRST------

General information: TITO was launched using: RESULT: Template: 2F86.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 442 14303 32.36 121.21 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 32.36 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.395

(partial model without unconserved sides chains): PDB file : Tito_2F86.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F86-query.scw PDB file : Tito_Scwrl_2F86.pdb: