Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTIFKQKNTHPFSNAANRLDRLPISRVHFQVLTALGIVYFFDLADLFTLSNVAPALIEHWGIPLSTIANVTAASFLGMFLGASLGGRLSDRIGRKKALNLFVFVFSIASLCNAAAWDIPSLMTFRFLTGFGVAAAMVITNSYLAEFFPSSVRGKYISFCAMIGLIGVPITNIVSAFVIPLGSWGWRLVFVWGAVGLIYFFF-IHRLEESPRWHENRGEYAKADAILTRIEEQVEKEKGPLPAASQPKVSETVKQNAGYAGLLKGRNLKI-TIVLSAVWIFET-FGFYGFASWVPSLLKSNGVTMENTLWYNVLHSVGAPLGALLGSMISERFQRKWILAASAFLTAIAGLLYGM-------TFIPIMII----VFGFIVNITERVFTSNLYAYTSEPYPTEYRSSGSGLAYGLGRFSNIFGSLLVGFIAVQLGYISVFLFIGGCWLACSLLLIFFGPNTNAKQI 4LDS Chain:A ((7-425)) ------------------------------LIFILGALGGLLYGYDNGVISGALLFIHKDIPLNSTTEGIVVSSMLIGAIVGAGSSGPLADKLGRRRLVMLIAIVFIIGALILAASTNLALLIIGRLIIGLAVGGSMSTVPVYLSEMAPTEYRGSLGSLNQLMITIGILAAYLVNYAFAD--IEGWRWMLGLAVVPSVILLVGIYFMPESPRWLLENRNEEAARQVM-KITYDDSEIDKELK---EMKE-INAISEST-WTVIKSPWLGRILIVGCIFAIFQQFIGINAVIFYSSSIFAKAGLGEAASILGSVGIGTINVLVTIVAIFVVDKIDRKKLLVGGNIGMIASLLIMAILIWTIGIASSAWIIIVCLSLFIVFFGIS---WGPVLWVMLPELFPMRARGAATGISALVLNIGTLIVSLFFPILSDALSTEWVFLIFAFIGVLAMIFVIKFLPET-----

General information: TITO was launched using: RESULT: Template: 4LDS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2121 -267640 -126.19 -660.84 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -126.19 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.315

(partial model without unconserved sides chains): PDB file : Tito_4LDS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LDS-query.scw PDB file : Tito_Scwrl_4LDS.pdb: