Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MPWSMKDYPASLKNLEKPVRK---KAIDIANAMIDEGYEEGRAIPIATSKAKEWAENASTDEIDDFLTHDDETERDADPSSG--SGPELMNKAEHVIKHKKGWAVKAEGAKRV-------------SEIKDTKKEAIERAKEIAA---HKGTEVIVHLADGSVQRKIKTGS------------------------- 4U6N Chain:A ((8-200)) NVQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKILCCGNG----TSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQVRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVEIRIPSHRSARIQEMHMLTVNCLCDLIDNTLFP

General information: TITO was launched using: RESULT: Template: 4U6N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 435 -15017 -34.52 -102.85 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -34.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.205

(partial model without unconserved sides chains): PDB file : Tito_4U6N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U6N-query.scw PDB file : Tito_Scwrl_4U6N.pdb: