Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MRKKRVITCVMAASLTLGSLLPAGYASAKEDSKTTPSYEELALHYKMKSEKISSNGKLVEIEYVSGNETHKVQMNGNNHTVKVDGIEQKGLNFEYDENVAKRTNYENNNLKSNEFTTQAAKPKKGYHYVGTLSGHTKAAKNALSVTMSLVGIVPGLGWGSKAATILFSYWAKEQIPDAYYKYDLYEKGAMTDSWYQYATVQFFEDKAHKKKMGKPWTSTPAKVDLPNS 1K8K Chain:F ((2-167)) TATLRPYLSAVRATLQAALCLENF----------SSQVVER------HNKPEVEVRSSKELLLQPVTISRNEKEKVLIEGSINSVRV-SIAVKQADEIEKILCHKFMRFMMM--RAENFFILRRKPVEGYD-----------------------------------ISFLITNFHTEQMY----------KHKLVDFVIHF--MEEIDKEISEMKLSVNARARIVAEEFLKN

General information: TITO was launched using: RESULT: Template: 1K8K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 611 -46115 -75.47 -284.66 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain F : 0.61 3D Compatibility (PKB) : -75.47 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_1K8K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1K8K-query.scw PDB file : Tito_Scwrl_1K8K.pdb: