Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDFAFKLYEYNVWANQQIFNRLKELPKEIYHQEIQSVFPSISHVLSHVYLSDLGWIEVFSGKTL---SDAL-A----LAEQ-----LKEQTEAKEIEEMEDLFLRLSERYILFLQQ--KEQLNKPLQIQNPSSGIMKTTVSELLPH-VVNHGTYHRGNITAMLRQAGYASAPTDYGLYLFMTKTEKA 5COG Chain:A ((18-182)) ---SKKELKEEIEKKYEKYDAEFETISESQKDEKVETVDRTPSENLSYQLGWVNLLLEWEAKEIAGYNVETPAPGYKWNNLGGLYQSFYKKYGIYSIKEQRAKLREAVNEVYKWISTLSDDELFQAGNRKWA-TTKAMWPVYKWIHINTVAPFTNFRGKIRKWKRLVPE-------------------

General information: TITO was launched using: RESULT: Template: 5COG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 553 -36689 -66.34 -246.23 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -66.34 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_5COG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5COG-query.scw PDB file : Tito_Scwrl_5COG.pdb: