Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTILAIDTSNYTLGIALLREDTVIAEYITYLKKNHSVRAMPAVHSLLNDCDMAPQDLSKIVVAKGPGSYTGVRIGVTLAKTLAWSLDIPISAVSSLETLAANGRHFDGL--ISPIFDARRGQVYTGLYQY-KNGLLEQVVPDQNVMLADWLEMLKEKDRPVLFLGHDTSLHKQMIEDVLGTKGFIGTAAQHNPRPSELAFLGKEKEAADVHGLVPDYLRLAEAEAKWIESQK 4WQ5 Chain:D ((1-134)) MRILAIDTATEACSVALWNDGTVNAHF-ELCPREHTQRILPMVQDILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGAWRKNGATRVLAAIDARMGEVYWAEYQRDENGI-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4WQ5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 563 -90183 -160.18 -688.42 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain D : 0.70 3D Compatibility (PKB) : -160.18 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_4WQ5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WQ5-query.scw PDB file : Tito_Scwrl_4WQ5.pdb: