Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKKWQWLVYGWKLIILTLLTAAVFSYAMFQGGFVSWFLFYAFLPFVLYAGLLALYPLRSFQASRQMDKTQLHAGDRLGVTVTLRRKLPFPLMYMVIEDCLPEGIESLNRDGAAAKRLVFPWFKRSMTMSYELARVPRGEHHFHSVRVRTGDVLGLIEKTAFFELDDTLFVYPFYQRFSYQVNERHQEDGVSGSSPIHQHHSSVAASVRNYQPGDRFAALDWKTSARRSQLMTKEFEPSRSKNLFLLMDRFSSDAFEEVVSVTASILHSVLKNGAGAGLASIGKEKNIFPIQEGDQHFKHMLRHLAIAHCDAADPISRYAREELGKPSVRQADQVVVTGQLTEDMLHLAEIGGGRVTVILAKEKDAELSQAENVMIERMMKRQIRVRIMRGGRVSRVV 1L9M Chain:A ((595-671)) -------------------------------------------------------------TLTLEVL-NEARVRKPVNVQMLFSNPLDEPVRDCVLMVEGSGLLLGN-----LKIDVPTLGPKERSRVRFDILPSRSGTKQLL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1L9M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 275 -33333 -121.21 -432.89 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : -121.21 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_1L9M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L9M-query.scw PDB file : Tito_Scwrl_1L9M.pdb: