Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTLTVKDIKDRLQEVKDAQDPFIAQCENDPRKSVQTLVEQWLKKQAKEKALKEQWVNMTSYERLARNKGFRLIAGVDEVGRGPLAGPVVASAVILPEE----CEILGLTDSKKLSEKKREEYYELIMKEALAVGIGIVEATVIDEINIYE--ASKMAMVKAIQDLSDTPDYLLVDAMTLP---L----------DTAQASIIKGDAKSVSIAAGACIAKVTRDRMMSAYAETYPMYGFEKNKGYGTKEHLEA-LAAYGP-TELHRKTFAPVQSFR 1UAX Chain:A ((2-204)) ------------------------------------------------------------------------KVAGVDEAGRGPVIGPLVIGVAVIDEKNIERLRDIGVKDSKQLTPGQREKLFSKLIDILDDYYVLLVTPKEIDERHHSMNELEAEKFVVALNSLRIKPQKIYVDSADVDPKRFASLIKAGLKYEATVIAEHKADAKYEIVSAASIIAKVTRDREIEKLKQKYGEFGSGYPSDPRTKEWLEEYYKQYGDFPPIVRRTWETARKI-

General information: TITO was launched using: RESULT: Template: 1UAX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 905 -79797 -88.17 -438.44 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -88.17 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.414

(partial model without unconserved sides chains): PDB file : Tito_1UAX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UAX-query.scw PDB file : Tito_Scwrl_1UAX.pdb: