Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MDIIR--KISHFAGQTFGIWVI----------------VFAVLGFSFPSLFTWISSY---------ITIFLGIIMFGMGLTLQADD----FKELVRKPWQVIIGVIAQYTIMPLVAFGLAFGLHLPAEIAVGVILVGCCPGG-----TASNVMTFLAKGNTALSVAVTTISTLLAPVVTPLLIMLFAKEWLPVSPGSLFISILQAVLFPIIAGLIVKMFFRKQVAKAVHALPLVSVIGIVAIVSAVVSGNRENLLQSGLLIFSVVILHNGIGYLLGFLCAKLLKMDYPSQKAIAIEVGMQNSGLGAALATAHFSPLSAVPSAIFSVWHNLSGSMLATYWSKKVKKKQA---------GSKSSNLSL-------- 2CY7 Chain:A ((5-377)) TLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQP-DSYFSVLNAFIDRK----DSYYSIHQIAQMGVGE---GKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIA---MDNTVVMEEIRRLCRTSVPCSPWRP-----LVLLIPLRLGL--TDINEAYVETLKHCFMMPQSLGVI--------GGKPN----------------SAHYFIGYVGEELIYLDPHTTQPAVEGCFIPDESFHCQHPPCRMSIAELDPSIAVGFFCK--TEDDFNDWCQQVKKLSLLPMFELVEQQPDVLNLSLDSSDVERL

General information: TITO was launched using: RESULT: Template: 2CY7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1367 -87907 -64.31 -317.35 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -64.31 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_2CY7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CY7-query.scw PDB file : Tito_Scwrl_2CY7.pdb: