Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIYHMKVRTITGKDMTLQPFAGKVLMIVNTASKCGFT-SQLKQLQELYDTYQQEGLEILGFPCNQFMNQEPGEEADIQEFCETNYGVTFPMFSKVDVNGKNAHPLFVYLTEHAKGMLGTKAIKWNFTKFIVDRNGEIVGRYSPNTNPKELEDDIVKLLEQ 2P5Q Chain:B ((11-168)) -SVHDFTVKDAKENDVDLSIFKGKVLLIVNVASKCGMTNSNYAEMNQLYEKYKDQGLEILAFPCNQFGEEEPGTNDQITDFVCTRFKSEFPIFDKIDVNGENASPLYRFLKLGKWGIFGDD-IQWNFAKFLVNKDGQVVDRYYPTTSPLSLERDIKQLLE-

General information: TITO was launched using: RESULT: Template: 2P5Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 789 -11262 -14.27 -71.73 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -14.27 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_2P5Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P5Q-query.scw PDB file : Tito_Scwrl_2P5Q.pdb: