Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------MFSPQQPMRNYPQPGPPRPMPNQRMMGRRPPNMRGPSFGAQQQGFQQAGQFLPQANAG------ARNGAGAGGGLKGMLSRFLPGGGGAGSAGVPG-IPGAGAAASGGAGLQGIQNIANPASLSSMLGNVQKVLG-----MAQQVTPMIQQYGPLV---------RNLPAMMKLYS------------QLSKSDDTETEANEESEKQSVSEESSEKEK------ETETKTSDGNKKSKPKSSSLPKKSKTTDNKEQELKTSAPKKKEAASPAPKRTS------GSSKPRLYI 3R4V Chain:A ((2-315)) PVKVCLIFAGGTGMNVATKLVDLGEAVHCFDTCDKNVVDVHRSVNVTLTKGTRGNRKVILPLVRPQIPALMDTIPEADFYIVCYSLGGGSGSVLGPLITGQLADRKASFVSFVVGAMESTDNLGNDIDTMKTLEAIAVNKHLPIVVNYVPNTQGRSYESINDEIAEKIRKVVLLVNQNHGRLDVHDVANWVRFTDKHNYLIPQVCELHIETTRKDAENVPEAISQLSLYLDPSKEVAFGTPIYRKVGIMKVDDLDVTDDQIHFVINSVGVVEIMKTITDSKLEMTRQQSKFTQRNPIIDADDNVDEDGMVV

General information: TITO was launched using: RESULT: Template: 3R4V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1312 85686 65.31 346.90 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 65.31 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.174

(partial model without unconserved sides chains): PDB file : Tito_3R4V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R4V-query.scw PDB file : Tito_Scwrl_3R4V.pdb: