Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKWSELSIHTTHEAVEPISNILHEAGASGVVIEDPLDLIKERENVYGEIYQLDPNDYPDEGVIVKAYLPVNSFLGETVDGIKETINNLLLYNIDLGRNHITISEVNEEEWATAWKKYYHPVKISEKFTIVPTWEEYTPVHTDELIIEMDPGMAFGTGTHPTTVLCIQALERFVQKGDKVIDVGTGSGILSIAAAMLEAESVHAYDLDPVAVESARLNLKLNKVSDIAQVKQNNLLDGIE-GEHDVIVANILAEVILRFTSQAYSLLKEGGHFITSGIIGHKKQEVKEALEQAGFTIVEILSMEDWVSIIAKK 3CJT Chain:M ((53-248)) -------------------------------------------------------------------------------------------------------EVGDEDWLEAWRRDLKPA-LAPPFVVLAPWHTW---EGAEIPLVIEPGMAFGTGHAETTRLALKALARHLRPGDKVLDLGTGSGVLAIAAEKLGGKAL-GVDIDPMVLPQAEANAKRNGVRP--RFLEGSLEAALPFGPFDLLVANLYAELHAALAPRYREALVPGGRALLTGILKDRAPLVREAMAGAGFRPLEEAAEGEWV------

General information: TITO was launched using: RESULT: Template: 3CJT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 1043 -70714 -67.80 -362.64 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain M : 0.68 3D Compatibility (PKB) : -67.80 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_3CJT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CJT-query.scw PDB file : Tito_Scwrl_3CJT.pdb: