Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMAKKCSVFMLCAAVSLSLAACGPKESSSAKSSSKGSELVVWEDKEKSNGI-KDAVAAFEKEHDVKVKVVEKPYAKQIEDLRMDGPAGTGPDVLTMPGDQIGTAVTEGLLKELHVKKDVQSLYTDASIQSQMVDQKLYGLPKAVETTVLFYNKDLITEKELPKTLEEWYDYSKKTADGSKFGFLALFD--QIYYAESVMSGYGGYIFGKAKDGS-YNPSDIGINNEGAVKGAALIQKFYKDGLFPAGIIGEQGINVLESLFTEGKAAAIISGPWNVEAFSNA-GINYGITKLPKLENGKNMSSFIGVKSYNVSAFSKNEELAQELAVFLANEKNSKTRYEETKEVPA-VKSLANDPAIMKSEAARAVTEQSRFSEPTPNIPEMNEIWTPADSALQTVATGKADPKQALDQAAETAKGQIKAKHSGK 4HW8 Chain:B ((37-411)) ---------------------------------KDKPNQLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPAGKGPDIFFLAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNKKLV--KNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIF--KKNGSEYDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHD----VMIGLFKEGKVGQFVTGPWNINEYQETFGKDLGVTTLP-TDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQKYTDEMSEITGRVDVKSSNPNL------KVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNGK-NPKQALDEATNDITQNIKILH---

General information: TITO was launched using: RESULT: Template: 4HW8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2030 115295 56.80 312.45 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : 56.80 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_4HW8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HW8-query.scw PDB file : Tito_Scwrl_4HW8.pdb: