Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------MNHLPEKMRPYRDLLEKSAKEYVKL---------------------NVRKGKTGRYDSKIAGDPYFPKHETYPTD----ENGQPMKLLAQINFSHIPQLDGY----------PSSGILQFYISVHDDVYGLNFDDRCEQKNFRVIY---------FENIVENDDELVSDFSFIGTGECDFPILSEAAVEPVKSSEWVLPTDFQFEQYTGMETMEFFGQFGEDEEDIYNELAENGFGHKIGGYASFTQHDPREYAYKEHTIMLLQIDS----------------------DDDIDSMWGDVGIANFFITPEDLRKKDFSNVLYNWDCS---------- 1KFU Chain:L ((2-355)) AGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGMRWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDIT--SAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSDF-LRHYSRLEICNLTPDTLTSDTYKK

General information: TITO was launched using: RESULT: Template: 1KFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 1117 49076 43.94 190.22 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain L : 0.62 3D Compatibility (PKB) : 43.94 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_1KFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KFU-query.scw PDB file : Tito_Scwrl_1KFU.pdb: