Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MNYEIFKAIHGLSHHNSVLDSIMVFITEYAIVAYALILL------------------------------AIWLFGNTQSRKHVLYAGITGIAGLVINYLITLV--YFEPRPFVAHTVHTLIPHAADASFPSDHTTGALAISIAMLFRNRKIGWPLVIFGLL-------TGFSRIWVGHHYPVDVLGSLVVAIIIGFLFFRFSDLLRPFVDLVVRIYEAIINKLTKKPTDQNF------------------------------------ 4DMZ Chain:A ((22-321)) NDQSLRSSLLGLRQLLRELPGDEAPLDALAETVLALLAQYGSLRIAGLYRVRHDRTPEPQPLATLGEMPVLDADDLLVRTCLERGELVSVRALQVCVPLVDTDGRILALLAVEQMPFFVFNERTFSLLAILAGHIADL-LQDIDAQRFSQYLKRSLLDARDHGLPACLYAFELTDARYGEEVQRLLEGSQRGLDVQLRLRNDEGRRVLLVLLPLTSAEGSQGYLQRLRILFAERFGQARELESLGVRIRQYELDAGNDRQALGHFLFNECGLNDQQVAV

General information: TITO was launched using: RESULT: Template: 4DMZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 770 -76218 -98.98 -396.97 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -98.98 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_4DMZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DMZ-query.scw PDB file : Tito_Scwrl_4DMZ.pdb: